Anton V.
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Bio- and cheminformatics
Plasmid Map Generator
Plasmid Map Generator

Create simple and clean plasmid maps from GenBank files.
Plasmid in the GenBank flat file format (.gb)
LOCUS       Exported                7700 bp ds-DNA     circular SYN 23-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   brady
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 10, 2021 from SnapGene 5.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 orim
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             599..1459
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2404..2546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2648..2839)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3648..4730)
                     /direction=RIGHT
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4731..4808)
                     /direction=RIGHT
                     /gene="lacI"
                     /label=lacI promoter
     promoter        5121..5139
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5140..5164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5209..5535
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             5557..5574
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5578..5628)
                     /label=AviTag Insert R
     CDS             5584..5604
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV Site
                     /translation="ENLYFQG"
     CDS             5611..5655
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     5629..5673
                     /label=AviTag Insert F
     CDS             5659..7503
                     /codon_start=1
                     /label=dsnPPR10-C2
                     /translation="MGNDVVTYTTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNT
                     LIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYTTLIDGLAKAGRLEEALCLFQEMKEK
                     GVKPNVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLAKAGRLEE
                     ALQLFQEMKEKGVKPSVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPSVVTYNTLI
                     DGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGV
                     KPDVVTYTTLIDGLAKAGRLEEALQLFQEMKEKGVKPNVVTYTTLIDGLAKAGRLEEAL
                     QLFQEMKEKGVKPNVVTYNTLIDGLAKAGRLEEALCLFQEMKEKGVKPSVVTYNTLIDG
                     LAKAGRLEEALQLFQEMKEKGVKPSVVTYTTLIDGLAKAGRLEEALQLFQEMKEKGVKP
                     NVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLAKAGRLEEALQL
                     FQEMKEKGVKPDVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLA
                     KAGRLEEALQLFQEMKEKGVKPSVVTNNTLKDGASKAG"
     CDS             7544..7561
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7628..7675
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
     3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
     3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
     3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
     3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
     3901 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta
     3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
     4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
     4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
     4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
     4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
     4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
     4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
     4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
     4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
     4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
     4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
     4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
     4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
     4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
     4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
     5101 cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
     5161 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gagcgataaa
     5221 attattcacc tgactgacga cagttttgac acggatgtac tcaaagcgga cggggcgatc
     5281 ctcgtcgatt tctgggcaga gtggtgcggt ccgtgcaaaa tgatcgcccc gattctggat
     5341 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcaaaac
     5401 cctggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac
     5461 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc
     5521 gacgctaacc tggccggttc tggttctggc catatgcacc atcatcatca tcattcttct
     5581 ggtgagaatc tttattttca gggcgggtcc gggttgaatg atattttcga agcacagaaa
     5641 attgaatggc atgaggccat gggcaacgat gtggttacct acaccaccct gatcgatggt
     5701 ctggcgaaag cgggccgtct ggaggaagcg ctgcagctgt tccaagagat gaaggaaaaa
     5761 ggtgtgaagc cggacgtggt tacctataac accctgattg acggtctggc gaaggctggc
     5821 cgtctggagg aagcgctgca actgtttcag gaaatgaagg aaaaaggcgt taaaccggat
     5881 gttgtgacct ataccaccct gattgatggt ctggcgaagg ccggccgtct ggaggaagcg
     5941 ctgtgcctgt tccaggagat gaaagagaaa ggtgtgaagc cgaatgttgt gacctacaat
     6001 accctgatcg acggtctggc gaaggcaggc cgtctggagg aagcgctgca gttatttcag
     6061 gagatgaaag aaaaaggcgt taagccggat gttgttacct acaacacttt aattgacggt
     6121 ctggcgaaag ctggccgtct ggaggaagcg ctgcagctat ttcaggagat gaaggagaaa
     6181 ggtgtgaaac cgagcgtggt gacctacaac actttaatcg atggtctggc gaaggcgggc
     6241 cgtctggagg aagcgctgca gctttttcag gagatgaagg aaaaaggcgt taagccgagc
     6301 gtggttacct acaacactct tattgatggt ctggcgaaag ctggccgtct ggaggaagcg
     6361 ctgcagctct ttcaggagat gaaggaaaaa ggtgtgaaac cggatgttgt tacctataat
     6421 actttgatag atggtctggc gaaggcgggc cgtctggagg aagcgctgca gctattccag
     6481 gaaatgaaag agaaaggcgt taaaccggac gttgtgacct acaccacttt aattgatggt
     6541 ctggcgaaag ccggccgtct ggaggaagcg ctgcagctat tccaagaaat gaaagaaaaa
     6601 ggtgtgaagc ctaacgtggt tacctatacc actttaatcg acggtctggc gaaagccggc
     6661 cgtctggagg aagcgctgca gctattccaa gagatgaaag agaaaggcgt taagccgaat
     6721 gtggtgacct acaacacatt aattgatggt ctggcgaaag ccggccgtct ggaggaagcg
     6781 ctgtgtctgt tccaggagat gaaggaaaaa ggtgtgaagc cgagcgtggt gacctataat
     6841 actttgatcg atggtctggc gaaagcaggc cgtctggagg aagcgctgca gctatttcaa
     6901 gaaatgaaag aaaaaggcgt taagccgagc gtggtaacct ataccactct tatcgacggt
     6961 ctggcgaaag caggccgtct ggaggaagcg ctgcagctat ttcaagagat gaaagaaaaa
     7021 ggtgtgaaac ctaatgttgt gacctacaac actcttatcg atggtctggc gaaagcaggc
     7081 cgtctggagg aagcgctgca gctatttcaa gagatgaagg agaaaggcgt taagcctgat
     7141 gtggttacct ataatactct catcgatggt ctggcgaaag caggccgtct ggaggaagcg
     7201 ctgcagctat ttcaagagat gaaggaaaaa ggtgtgaagc cagatgttgt gacctataat
     7261 actctaatag atggtctggc gaaggcgggc cgtctggagg aagcgctgca gctatttcaa
     7321 gagatgaagg aaaaaggtgt taaaccggat gtggttacct acaacacact tattgatggt
     7381 ctggcgaaag caggccgtct ggaggaagcg ctgcagctat ttcaagagat gaaggaaaag
     7441 ggtgtgaaac cgagcgttgt gaccaataac accctgaagg acggcgcgag caaagcgggc
     7501 taagaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
     7561 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     7621 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     7681 aggaggaact atatccggat
//1
LOCUS       Exported                7700 bp ds-DNA     circular SYN 23-MAR-2018
2
DEFINITION  synthetic circular DNA
3
ACCESSION   .
4
VERSION     .
5
KEYWORDS    .
6
SOURCE      synthetic DNA construct
7
  ORGANISM  synthetic DNA construct
8
REFERENCE   1  (bases 1 to 7700)
9
  AUTHORS   brady
10
  TITLE     Direct Submission
11
  JOURNAL   Exported Wednesday, Mar 10, 2021 from SnapGene 5.2.1
12
            https://www.snapgene.com
13
FEATURES             Location/Qualifiers
14
     source          1..7700
15
                     /organism="synthetic DNA construct"
16
                     /mol_type="other DNA"
17
     rep_origin      12..467
18
                     /direction=RIGHT
19
                     /label=f1 orim
20
                     /note="f1 bacteriophage origin of replication; arrow 
21
                     indicates direction of (+) strand synthesis"
22
     promoter        494..598
23
                     /gene="bla"
24
                     /label=AmpR promoter
25
     CDS             599..1459
26
                     /codon_start=1
27
                     /gene="bla"
28
                     /product="beta-lactamase"
29
                     /label=AmpR
30
                     /note="confers resistance to ampicillin, carbenicillin, and
31
                     related antibiotics"
32
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
33
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
34
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
35
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
36
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
37
                     LIKHW"
38
     rep_origin      1630..2218
39
                     /direction=RIGHT
40
                     /label=ori
41
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
42
                     replication"
43
     misc_feature    2404..2546
44
                     /label=bom
45
                     /note="basis of mobility region from pBR322"
46
     CDS             complement(2648..2839)
47
                     /codon_start=1
48
                     /gene="rop"
49
                     /product="Rop protein, which maintains plasmids at low copy
50
                     number"
51
                     /label=rop
52
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
53
                     DELYRSCLARFGDDGENL"
54
     CDS             complement(3648..4730)
55
                     /direction=RIGHT
56
                     /codon_start=1
57
                     /gene="lacI"
58
                     /product="lac repressor"
59
                     /label=lacI
60
                     /note="The lac repressor binds to the lac operator to 
61
                     inhibit transcription in E. coli. This inhibition can be 
62
                     relieved by adding lactose or 
63
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
64
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
65
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
66
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
67
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
68
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
69
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
70
                     ALADSLMQLARQVSRLESGQ"
71
     promoter        complement(4731..4808)
72
                     /direction=RIGHT
73
                     /gene="lacI"
74
                     /label=lacI promoter
75
     promoter        5121..5139
76
                     /label=T7 promoter
77
                     /note="promoter for bacteriophage T7 RNA polymerase"
78
     protein_bind    5140..5164
79
                     /label=lac operator
80
                     /bound_moiety="lac repressor encoded by lacI"
81
                     /note="The lac repressor binds to the lac operator to 
82
                     inhibit transcription in E. coli. This inhibition can be 
83
                     relieved by adding lactose or 
84
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
85
     CDS             5209..5535
86
                     /codon_start=1
87
                     /gene="trxA"
88
                     /product="E. coli thioredoxin"
89
                     /label=TrxA
90
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
91
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
92
                     DANLA"
93
     CDS             5557..5574
94
                     /codon_start=1
95
                     /product="6xHis affinity tag"
96
                     /label=6xHis
97
                     /translation="HHHHHH"
98
     primer_bind     complement(5578..5628)
99
                     /label=AviTag Insert R
100
     CDS             5584..5604
101
                     /codon_start=1
102
                     /product="tobacco etch virus (TEV) protease recognition and
103
                     cleavage site"
104
                     /label=TEV Site
105
                     /translation="ENLYFQG"
106
     CDS             5611..5655
107
                     /codon_start=1
108
                     /product="peptide tag that allows for enzymatic 
109
                     biotinylation"
110
                     /label=AviTag(TM)
111
                     /translation="GLNDIFEAQKIEWHE"
112
     primer_bind     5629..5673
113
                     /label=AviTag Insert F
114
     CDS             5659..7503
115
                     /codon_start=1
116
                     /label=dsnPPR10-C2
117
                     /translation="MGNDVVTYTTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNT
118
                     LIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYTTLIDGLAKAGRLEEALCLFQEMKEK
119
                     GVKPNVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLAKAGRLEE
120
                     ALQLFQEMKEKGVKPSVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPSVVTYNTLI
121
                     DGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGV
122
                     KPDVVTYTTLIDGLAKAGRLEEALQLFQEMKEKGVKPNVVTYTTLIDGLAKAGRLEEAL
123
                     QLFQEMKEKGVKPNVVTYNTLIDGLAKAGRLEEALCLFQEMKEKGVKPSVVTYNTLIDG
124
                     LAKAGRLEEALQLFQEMKEKGVKPSVVTYTTLIDGLAKAGRLEEALQLFQEMKEKGVKP
125
                     NVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLAKAGRLEEALQL
126
                     FQEMKEKGVKPDVVTYNTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLA
127
                     KAGRLEEALQLFQEMKEKGVKPSVVTNNTLKDGASKAG"
128
     CDS             7544..7561
129
                     /codon_start=1
130
                     /product="6xHis affinity tag"
131
                     /label=6xHis
132
                     /translation="HHHHHH"
133
     terminator      7628..7675
134
                     /label=T7 terminator
135
                     /note="transcription terminator for bacteriophage T7 RNA 
136
                     polymerase"
137
ORIGIN
138
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
139
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
140
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
141
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
142
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
143
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
144
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
145
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
146
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
147
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
148
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
149
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
150
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
151
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
152
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
153
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
154
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
155
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
156
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
157
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
158
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
159
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
160
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
161
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
162
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
163
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
164
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
165
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
166
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
167
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
168
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
169
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
170
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
171
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
172
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
173
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
174
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
175
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
176
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
177
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
178
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
179
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
180
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
181
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
182
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
183
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
184
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
185
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
186
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
187
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
188
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
189
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
190
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
191
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
192
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
193
     3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
194
     3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
195
     3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
196
     3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
197
     3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
198
     3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
199
     3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
200
     3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
201
     3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
202
     3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
203
     3901 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta
204
     3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
205
     4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
206
     4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
207
     4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
208
     4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
209
     4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
210
     4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
211
     4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
212
     4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
213
     4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
214
     4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
215
     4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
216
     4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
217
     4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
218
     4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
219
     4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
220
     4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
221
     4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
222
     5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
223
     5101 cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
224
     5161 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gagcgataaa
225
     5221 attattcacc tgactgacga cagttttgac acggatgtac tcaaagcgga cggggcgatc
226
     5281 ctcgtcgatt tctgggcaga gtggtgcggt ccgtgcaaaa tgatcgcccc gattctggat
227
     5341 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcaaaac
228
     5401 cctggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac
229
     5461 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc
230
     5521 gacgctaacc tggccggttc tggttctggc catatgcacc atcatcatca tcattcttct
231
     5581 ggtgagaatc tttattttca gggcgggtcc gggttgaatg atattttcga agcacagaaa
232
     5641 attgaatggc atgaggccat gggcaacgat gtggttacct acaccaccct gatcgatggt
233
     5701 ctggcgaaag cgggccgtct ggaggaagcg ctgcagctgt tccaagagat gaaggaaaaa
234
     5761 ggtgtgaagc cggacgtggt tacctataac accctgattg acggtctggc gaaggctggc
235
     5821 cgtctggagg aagcgctgca actgtttcag gaaatgaagg aaaaaggcgt taaaccggat
236
     5881 gttgtgacct ataccaccct gattgatggt ctggcgaagg ccggccgtct ggaggaagcg
237
     5941 ctgtgcctgt tccaggagat gaaagagaaa ggtgtgaagc cgaatgttgt gacctacaat
238
     6001 accctgatcg acggtctggc gaaggcaggc cgtctggagg aagcgctgca gttatttcag
239
     6061 gagatgaaag aaaaaggcgt taagccggat gttgttacct acaacacttt aattgacggt
240
     6121 ctggcgaaag ctggccgtct ggaggaagcg ctgcagctat ttcaggagat gaaggagaaa
241
     6181 ggtgtgaaac cgagcgtggt gacctacaac actttaatcg atggtctggc gaaggcgggc
242
     6241 cgtctggagg aagcgctgca gctttttcag gagatgaagg aaaaaggcgt taagccgagc
243
     6301 gtggttacct acaacactct tattgatggt ctggcgaaag ctggccgtct ggaggaagcg
244
     6361 ctgcagctct ttcaggagat gaaggaaaaa ggtgtgaaac cggatgttgt tacctataat
245
     6421 actttgatag atggtctggc gaaggcgggc cgtctggagg aagcgctgca gctattccag
246
     6481 gaaatgaaag agaaaggcgt taaaccggac gttgtgacct acaccacttt aattgatggt
247
     6541 ctggcgaaag ccggccgtct ggaggaagcg ctgcagctat tccaagaaat gaaagaaaaa
248
     6601 ggtgtgaagc ctaacgtggt tacctatacc actttaatcg acggtctggc gaaagccggc
249
     6661 cgtctggagg aagcgctgca gctattccaa gagatgaaag agaaaggcgt taagccgaat
250
     6721 gtggtgacct acaacacatt aattgatggt ctggcgaaag ccggccgtct ggaggaagcg
251
     6781 ctgtgtctgt tccaggagat gaaggaaaaa ggtgtgaagc cgagcgtggt gacctataat
252
     6841 actttgatcg atggtctggc gaaagcaggc cgtctggagg aagcgctgca gctatttcaa
253
     6901 gaaatgaaag aaaaaggcgt taagccgagc gtggtaacct ataccactct tatcgacggt
254
     6961 ctggcgaaag caggccgtct ggaggaagcg ctgcagctat ttcaagagat gaaagaaaaa
255
     7021 ggtgtgaaac ctaatgttgt gacctacaac actcttatcg atggtctggc gaaagcaggc
256
     7081 cgtctggagg aagcgctgca gctatttcaa gagatgaagg agaaaggcgt taagcctgat
257
     7141 gtggttacct ataatactct catcgatggt ctggcgaaag caggccgtct ggaggaagcg
258
     7201 ctgcagctat ttcaagagat gaaggaaaaa ggtgtgaagc cagatgttgt gacctataat
259
     7261 actctaatag atggtctggc gaaggcgggc cgtctggagg aagcgctgca gctatttcaa
260
     7321 gagatgaagg aaaaaggtgt taaaccggat gtggttacct acaacacact tattgatggt
261
     7381 ctggcgaaag caggccgtct ggaggaagcg ctgcagctat ttcaagagat gaaggaaaag
262
     7441 ggtgtgaaac cgagcgttgt gaccaataac accctgaagg acggcgcgag caaagcgggc
263
     7501 taagaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
264
     7561 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
265
     7621 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
266
     7681 aggaggaact atatccggat
267
//
The GenBank file of the plasmid to be rendered.
Label
The label of the plasmid to be rendered.
Plasmid map
The rendered plasmid map.
Plasmid Map Generator

See also
